Generator

Part:BBa_J29021:Design

Designed by: Mayu Ikezumi   Group: iGEM06_Chiba   (2006-08-03)


This is 4 enzymes that is used to produce a β-carotene.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1046
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 577
    Illegal NgoMIV site found at 707
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It uses a storong RBS.


Source

The 3 enzymes are CrtE(BBa_J29000), CreB(BBa_J29001), and CrtI(BBa_J29002). The 3 RBS BBa_B0030.

References